Bosch 480 Series Instrukcja Operatora Pobierz pdf (Strona 20)

LightCycler® 480 Gene Scanning Software

Gene Scanning with the LightCycler® 480 System

Components of the LightCycler® 480 System Gene Scanning Assay

The following examples demonstrate gene scanning:

For the single-nucleotide polymorphism (SNP) G ► →T in the LPLH3 gene (163 bp

amplicon), resulting in 3 main variant groups.

For sequence variations in the MBL2 gene (219 bp amplicon), resulting in 4 main vari- ►

ant groups corresponding to the 4 most frequent haplotypes described for this gene in

literature and 3 samples of a further genetic variant.

LightCycler2.3

480 Instrument

High Resolution Melting analysis of nucleic acid requires that the analyzing instrument

is able to detect even small changes in ﬂuorescence as a function of temperature-induced

DNA melting. In the LightCycler® 480 Instrument, images of DNA melting (i.e., ﬂuores-

cence) are captured by a sensitive CCD camera and magniﬁed to reveal subtle details in

DNA melting proﬁles. These proﬁles are then compared from sample to sample to deter-

mine relationships between the samples.

The optical components of the LightCycler® 480 System work together with its accurate

thermal control system to generate highly reproducible results from sample to sample.

Since the samples are held in a multiwell plate, the LightCycler® 480 System can analyze

many samples in a single run.

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Bosch 480 Series Instrukcja Operatora Strona 20